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Nucleotide polymorphisms and the 5′-UTR transcriptional analysis of the bovine growth hormone secretagogue receptor 1a (GHSR1a) gene

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Komatsu, M and Fujimori, Y and Sato, Y and Okamura, H and Sasaki, S and Itoh, T and Morita, M and Nakamura, R and Oe, T and Furuta, M and Yasuda, J and Kojima, T and Watanabe, T and Hayashi, T and Malau-Aduli, AEO and Takahashi, H (2010) Nucleotide polymorphisms and the 5′-UTR transcriptional analysis of the bovine growth hormone secretagogue receptor 1a (GHSR1a) gene. Animal Science Journal, 81 (5). pp. 530-550. ISSN 1344-3941

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Abstract

Growth hormone secretagogue receptor 1a (GHSR1a) mediates the different actions of its endogenous ligand, ghrelin. Ghrelin-GHSR is involved in many important functions that include growth hormone secretion and food intake. We evaluated the haplotype variety and characterized the microsatellite ((TG)n, 5′-UTR) and nucleotide polymorphisms of the bovine GHSR1a gene. The nucleotide sequencing of this gene (~6 kb) revealed 47 single nucleotide polymorphisms (SNPs), four indels and the microsatellite ((GTTT)n, Intron 1). The 19 haplotypes were constructed from all nucleotide viability patterns and were divided into three major groups. Four SNPs (L24V, nt456(G>A), D191N and nt667(C>T)) and DelR242 in Exon 1 and a haplotype block of approximately 2.2 kb (nt667(C>T) ~ nt2884 (A>G)) were found in Bos taurus breeds. Breed differences in allele frequencies of the two microsatellites, nt-7(C>A), L24V, and DelR242 loci were found (P < 0.005). A DelR242 was found in the Japanese Shorthorn (frequency: ~ 0.44), Japanese Brown, five European cattle breeds, the Philippine native cattle, but none detected in the Japanese Black or the Mishima island cattle. Additionally, 5′-rapid amplification of cDNA ends and RT-PCR analyses revealed that there were two different kinds of transcripts: spliced, without a microsatellite within 5′-UTR (GHSR1a); and non-spliced, with the microsatellite (GHSR1b).

Item Type: Article
Keywords: Cattle, GHSR, haplotype, nucleotide polymorphism, transcription.
Journal or Publication Title: Animal Science Journal
Page Range: pp. 530-550
ISSN: 1344-3941
Identification Number - DOI: 10.1111/j.1740-0929.2010.00797.x
Date Deposited: 23 Nov 2010 04:41
Last Modified: 18 Nov 2014 04:13
URI: http://eprints.utas.edu.au/id/eprint/10212
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