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Reassessing the Role of N-Hydroxytryptamine in Auxin Biosynthesis

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Tivendale, ND and Davies, NW and Molesworth, PP and Davidson, SE and Smith, JA and Reid, JB and Ross, JJ (2010) Reassessing the Role of N-Hydroxytryptamine in Auxin Biosynthesis. Plant Physiology, 154. pp. 1957-1965. ISSN 1532-2548

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Abstract

The tryptamine pathway is one of five proposed pathways for the biosynthesis of indole-3-acetic acid (IAA), the primary auxin in plants. The enzymes AtYUC1 (Arabidopsis thaliana), FZY (Solanum lycopersicum), and ZmYUC (Zea mays) are reported to catalyze the conversion of tryptamine to N-hydroxytryptamine, putatively a rate-limiting step of the tryptamine pathway for IAA biosynthesis. This conclusion was based on in vitro assays followed by mass spectrometry or HPLC analyses. However,there are major inconsistencies between the mass spectra reported for the reaction products. Here, we present mass spectral data for authentic N-hydroxytryptamine, 5-hydroxytryptamine (serotonin), and tryptamine to demonstrate that at least some of the published mass spectral data for the YUC in vitro product are not consistent with N-hydroxytryptamine. We also show that tryptamine is not metabolized to IAA in pea (Pisum sativum) seeds, even though a PsYUC-like gene is strongly expressed in these organs. Combining these findings, we propose that at present there is insufficient evidence to consider N-hydroxytryptamine an intermediate for IAA biosynthesis.

Item Type: Article
Keywords: IAA biosynthesis Auxin mass spectrometry N-hydroxytryptamine pea
Journal or Publication Title: Plant Physiology
Page Range: pp. 1957-1965
ISSN: 1532-2548
Identification Number - DOI: 10.1104/pp.110.165803
Additional Information: Copyright © 2010 by the American Society of Plant Biologists
Date Deposited: 16 Jun 2011 01:17
Last Modified: 18 Nov 2014 04:18
URI: http://eprints.utas.edu.au/id/eprint/11047
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