Due to routine maintenance, access to the Library Open Repository will be interrupted on the morning of Friday 12th February.
We apologise for any inconvenience.

Library Open Repository

Gene expression profiling identifies two regulatory genes controlling dormancy and ABA sensitivity in Arabidopsis seeds


Downloads per month over past year

Barrero, JM and Millar, AA and Griffiths, J and Czechowski, T and Scheible, WR and Udvardi, M and Reid, JB and Ross, JJ and Jacobsen, JV and Gubler, F (2010) Gene expression profiling identifies two regulatory genes controlling dormancy and ABA sensitivity in Arabidopsis seeds. the Plant Journal, 61. pp. 611-621. ISSN 0032-0935

[img] PDF
Barrero_et_al_2...pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.


Seed dormancy is a very important trait that maximizes the survival of seed in nature, the control of which can have important repercussions on the yield of many crop species. We have used gene expression profiling to identify genes that are involved in dormancy regulation in Arabidopsis thaliana. RNA was isolated from imbibed dormant (D) and after-ripened (AR) ecotype C24 seeds, and then screened by quantitative RT-PCR (qRT-PCR) for differentially expressed transcription factors (TFs) and other regulatory genes. Out of 2207 genes screened, we have identified 39 that were differentially expressed during the first few hours of imbibition. After analyzing T-DNA insertion mutants for 22 of these genes, two displayed altered dormancy compared with the wild type. These mutants are affected in genes that encode a RING finger and an HDZip protein. The first, named DESPIERTO, is involved in ABA sensitivity during seed development, regulates the expression of ABI3, and produces a complete loss of dormancy when mutated. The second, the HDZip (ATHB20), is expressed during seed germination in the micropylar

Item Type: Article
Keywords: Arabidopsis seed hormone ABA abscisic acid
Journal or Publication Title: the Plant Journal
Page Range: pp. 611-621
ISSN: 0032-0935
Identification Number - DOI: doi: 10.1111/j.1365-313X.2009.04088.x
Additional Information: The definitive published version is available online at: http://onlinelibrary.wiley.com
Date Deposited: 16 Jun 2011 00:14
Last Modified: 18 Nov 2014 04:18
Item Statistics: View statistics for this item

Repository Staff Only (login required)

Item Control Page Item Control Page