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Brassinosteroids, de-etiolation and the re-emerging art of plant hormone quantification

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Symons, GM and Reid, JB (2008) Brassinosteroids, de-etiolation and the re-emerging art of plant hormone quantification. Plant Signaling & Behavior, 3 (10). pp. 868-870. ISSN 1559-2316

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Abstract

An increase in the use of molecular techniques has provided a significant insight into the function of genes, and how they are regulated and interact. However, in the field of plant hormone physiology, the increased use of these techniques has been accompanied by a reduction in the direct measurement of plant hormone levels by physiochemical methods. Instead, the transcript (mRNA) levels of genes involved in hormone metabolism are often used to predict endogenous hormone levels. The validity of this approach was recently tested by comparing the expression of a range of genes involved in BR synthesis, catabolism and perception, with the actual endogenous BR levels in pea seedlings grown under different light conditions.1,2 Based on this comparison, we now argue that gene expression analysis alone is not always a reliable indicator of endogenous hormone levels.

Item Type: Article
Keywords: brassinosteroid, gene expression, hormone levels, de-etiolation
Journal or Publication Title: Plant Signaling & Behavior
Page Range: pp. 868-870
ISSN: 1559-2316
Identification Number - DOI: 10.4161/psb.3.10.6063
Additional Information: Copyright © 2008 Landes Bioscience
Date Deposited: 08 Jul 2011 05:41
Last Modified: 18 Nov 2014 04:20
URI: http://eprints.utas.edu.au/id/eprint/11370
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