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DNA as a Dietary Biomarker in Antarctic Krill, Euphausia superba

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Passmore, AJ and Jarman, SN and Swadling, KM and Kawaguchi, S and McMinn, A and Nicol, S (2006) DNA as a Dietary Biomarker in Antarctic Krill, Euphausia superba. Marine Biotechnology, 8. pp. 686-696.

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Abstract

The diet of Antarctic krill (Euphausia superba) has been studied using a variety of techniques, but current methods still suffer from problems that are difficult to solve. This study examined an alternative approach utilizing DNA as a prey biomarker. Methods were developed for the preservation, extraction, and identification of prey DNA from krill collected in the field. Group-specific polymerase chain reaction (PCR) was used to amplify diatom prey (Phylum: Bacillariophyta) and the results from DNA clone libraries were compared with microscopic diet analysis. DNA analysis was superior to microscopy for prey detection. However, differences in prey relative abundance estimates between the two techniques suggested some bias in the DNAbased estimates. Quantification showed that large amounts of prey DNA had been successfully preserved and extracted. Overall the results suggest that the application of DNA-based diet analysis to krill warrants further investigation, particularly for prey that are difficult to study using other methods.

Item Type: Article
Keywords: krill diet analysis — foodweb — trophic
Journal or Publication Title: Marine Biotechnology
Page Range: pp. 686-696
Identification Number - DOI: 10.1007/s10126-005-6088-8
Additional Information: The final publication is available at http://www.springerlink.com
Date Deposited: 24 Nov 2011 00:52
Last Modified: 18 Nov 2014 04:24
URI: http://eprints.utas.edu.au/id/eprint/12251
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