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Hydrogen/deuterium exchange on aromatic rings during atmospheric pressure chemical ionization mass spectrometry


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Davies, NW and Smith, JA and Molesworth, PP and Ross, JJ (2010) Hydrogen/deuterium exchange on aromatic rings during atmospheric pressure chemical ionization mass spectrometry. Rapid Communications in Mass Spectrometry, 24. pp. 1105-1110. ISSN 1097-0231

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It has been demonstrated that substituted indoles fully labelled with deuterium on the aromatic ring can undergo substantial exchange back to partial and even fully protonated forms during atmospheric pressure chemical ionisation (APCI) liquid chromatography/mass spectrometry (LC/MS). The degree of this exchange was strongly dependent on the absolute quantity of analyte, the APCI desolvation temperature, the nature of the mobile phase, the mobile phase flow rate and the instrument used. Hydrogen/deuterium (H/D) exchange on several other aromatic ring systems during APCI LC/MS was either undetectable (nitrobenzene, aniline) or extremely small (acetanilide) compared to the effect observed for substituted indoles. This observation has major implications for quantitative assays using deuterium-labelled internal standards and for the detection of deuterium- labelled products from isotopically labelled feeding experiments where there is a risk of back exchange to the protonated form during the analysis.

Item Type: Article
Journal or Publication Title: Rapid Communications in Mass Spectrometry
Page Range: pp. 1105-1110
ISSN: 1097-0231
Identification Number - DOI: 10.1002/rcm.4488
Additional Information: The definitive published version is available online at: http://www.interscience.wiley.com
Date Deposited: 10 Feb 2012 01:02
Last Modified: 18 Nov 2014 04:27
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