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Gibberellin 2-oxidation and the SLN gene of Pisum sativum

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Lester, DR and Ross, JJ and Smith, JJ and Elliott, RC and Reid, JB (1999) Gibberellin 2-oxidation and the SLN gene of Pisum sativum. The Plant Journal, 19 (1). pp. 65-73. ISSN 1365-313X

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Abstract

Two cDNAs encoding gibberellin 2-oxidases were
isolated from maturing pea seeds. The ®rst,
PsGA2ox1, was isolated by activity screening of a
Lambda-ZAP cDNA library excised into phagemid form
and expressed in Escherichia coli. The second,
PsGA2ox2, was obtained initially as a PCR product
using degenerate primers designed according to
conserved regions of plant 2-oxoglutarate-dependent
dioxygenases. E. coli heterologous expression products
of PsGA2ox1 and PsGA2ox2 converted GA1 to GA8,
as shown by HPLC-radiocounting, and gas
chromatography-MS. PsGA2ox1 converted GA20 to
GA29, but GA20 was a poor substrate for the
PsGA2ox2 expression product. Furthermore, PsGA2ox1
converted GA29 to GA29-catabolite at a low level of
ef®ciency while PsGA2ox2 did not catalyse this step.
A cDNA of PsGA2ox1 isolated from plants of
genotype sln contained a single base deletion which
was predicted to produce a truncated protein and
gibberellin 2-oxidase activity could not be
demonstrated from this cDNA. A 10 bp size difference
between the introns of the SLN and sln PsGA2ox1
genes was used to show co-segregation between the
SLN and sln phenotypes and the size of the PCR
products. PsGA2ox1 transcripts were more abundant
in cotyledons than in shoots, while the reverse was
the case for PsGA2ox2. The expression patterns of the
genes, together with the effects of the sln mutation,
indicate that PsGA2ox1 plays a major role in GA20
deactivation in both shoots and maturing seeds, while
the PsGA2ox2 gene might be important for GA1
deactivation in the shoot.

Item Type: Article
Journal or Publication Title: The Plant Journal
Page Range: pp. 65-73
ISSN: 1365-313X
Identification Number - DOI: 10.1046/j.1365-313X.1999.00501.x
Additional Information:

Copyright 1999 Blackwell Science Ltd

Date Deposited: 05 Oct 2012 01:10
Last Modified: 18 Nov 2014 04:43
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