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Temperature effects on the dynamics of gonad and oocyte development in captive wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone

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Grubert, MA and Ritar, AJ (2004) Temperature effects on the dynamics of gonad and oocyte development in captive wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone. Invertebrate Reproduction and Development, 45 (3). pp. 185-196. ISSN 0792-4259

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Abstract

Wild-caught blacklip (Haliotis rubra) and greenlip (H. laevigata) abalone were held
from spent condition at 12 degrees C, 14 degrees C, 16 degrees C or 18 degrees C and routinely sampled to examine gonad
development. Descriptors of gross structure included the Visual Gonad Index (VGI) and
the Modified Gonad Bulk Index (MGBI). Oocyte Diameter Ratio (ODR) and oocyte
volume (based on an ellipsoid) were used as descriptors of ovarian microstructure. For
each species, the rate of increase in the VGI, MGBI and oocyte volume of animals held at
different temperatures were used to estimate the Biological Zero Point (BZP), the critical
temperature below which no development occurs. BZP estimates derived from the daily
increase in VGI and oocyte volume were similar (7.8 degrees C and 7.6 degrees C for blacklip abalone;
6.9 degrees C and 6.8 degrees C for greenlip abalone, respectively), but those based on the increase in
MGBI were up to 1.8 degrees C lower (6.0 degrees C and 5.7 degrees C, for blacklip and greenlip abalone,
respectively). The mean MGBI, in terms of gonad volume per gram of shucked animal
weight, ranged from 5-68 mm3g-1 and 5-58 mm3g-1 for blacklip and greenlip abalone,
respectively. The ODR indicated that oocyte shape was highly variable in oocytes < 90 um
diameter in both species. Above 90 um, ODR values increased proportionally with oocyte
size, indicating a transition in shape from elliptical to round. Ranges for mean oocyte
volume for blacklip and greenlip abalone were 0.15-1.4 x 106 um3 and 0.02-1.83 x 106
um3, respectively. The pattern of oocyte growth relative to temperature for both species is
illustrated using tables of standardized residuals. Determination of the BZP for blacklip
and greenlip abalone enables the calculation of the Effective Accumulative Temperature
EAT; the cumulative difference between the water temperature and the BZP, calculated
daily) for gamete maturation of these species. This in turn facilitates predictive and
deductive estimates of the completion of this process (when water temperature is known)
in either natural or artificial (i.e. culture) environments.

Item Type: Article
Keywords: Biological zero point; Haliotis laevigata; Haliotis rubra; Modified gonad bulk index; Oocyte morphometrics; Temperature; Visual gonad index
Journal or Publication Title: Invertebrate Reproduction and Development
Page Range: pp. 185-196
ISSN: 0792-4259
Date Deposited: 20 Aug 2007
Last Modified: 18 Nov 2014 03:20
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