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First reports of Pseudo-nitzschia micropora and P. hasleana (Bacillariaceae) from the Southern Hemisphere: Morphological, molecular and toxicological characterization

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Ajani, P and Murray, S and Hallegraeff, GM and Brett, S and Armand, L (2013) First reports of Pseudo-nitzschia micropora and P. hasleana (Bacillariaceae) from the Southern Hemisphere: Morphological, molecular and toxicological characterization. Phycological Research, 61. pp. 237-248. ISSN 1322-0829

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Abstract

Pseudo-nitzschia H. Peragallo is a marine diatom
genus found worldwide in polar, temperate, subtropical
and tropical waters. It includes toxigenic representatives
that produce domoic acid (DA), a
neurotoxin responsible for Amnesic Shellfish Poisoning.
In this study we characterized two species of
Pseudo-nitzschia collected from Port Stephens and
the Hawkesbury River (south eastern Australia) previously
unreported from Australian waters. Clonal isolates
were sub-sampled for (i) light and transmission
electron microscopy; (ii) DNA sequencing, based on
the nuclear-encoded partial large subunit ribosomal
RNA gene and internal transcribed spacer (ITS)-ITS1,
5.8S and ITS2 rDNA regions and, (iii) DA production
as measured by liquid chromatography-mass spectrometry.
Morphological and molecular data unambiguously
revealed the species to be Pseudo-nitzschia
micropora Priisholm, Moestrup & Lundholm (Port
Stephens) and Pseudo-nitzschia hasleana Lundholm
(Hawkesbury River). This is the first report of the
occurrence of these species from the Southern Hemisphere
and the first report of P. micropora in warmtemperate
waters. Cultures of P. micropora, tested for
DA production for the first time, proved to be nontoxic.
Similarly, no detectable toxin concentrations
were observed for P. hasleana. Species resolution and
knowledge on the toxicity of local Pseudo-nitzschia
species has important implications for harmful algal
bloom monitoring and management.

Item Type: Article
Journal or Publication Title: Phycological Research
Page Range: pp. 237-248
ISSN: 1322-0829
Identification Number - DOI: 10.1111/pre.12020
Additional Information:

Copyright 2013 Japanese Society of Phycology

Date Deposited: 14 Oct 2013 04:53
Last Modified: 18 Nov 2014 04:55
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