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Further investigations of lupus anticoagulant interference in a functional assay for tissue factor pathway inhibitor

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Adams, MJ and Oostryck, R (1997) Further investigations of lupus anticoagulant interference in a functional assay for tissue factor pathway inhibitor. Thrombosis Research, 87 (2). pp. 245-249. ISSN 0049-3848

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Abstract

Tissue factor pathway inhibitor (TFPI) is a natural inhibitor that regulates the initiation of
coagulation by inhibiting tissue factor - activated factor VII (TF- FVIIa) in the presence of
activated factor X (FXa) (1). Although the precise role of TFPI in haemostasis is yet to be
determined, it's ability to inhibit TF - FVIla in the presence of FXa suggests that is does have an
important physiological significance.
TFPI levels have been reported to be normal in a variety of clinical conditions, including patients
with lupus anticoagulants (2,3). Lupus anticoagulants (LA) are acquired inhibitors, generally
considered to be immunoglobulins, that interfere with in vitro phospholipid dependent
coagulation tests (43). There is current interest in LA due to the associated thrombotic
complications as seen in increased risk for venous and arterial thrombosis as well as pregnancy
complications (6). We have previously reported decreased functional levels of TFPI in plasma
containing LA using a fluid phase assay, but normal levels with a binding assay (7). These low
levels of TFPI were shown to be corrected with the addition of excess TF (7).
As a continuation of this study, we again assessed TFPI levels in LA plasma using the same
fluid assay. The effect of using three different sources of tissue factor (recombinant, human
brain and rabbit brain thromboplastins) in the TFPI assay was examined. Of most significance
was the apparent correction of TFPI values as thromboplastin levels increased

Item Type: Article
Keywords: Tissue factor pathway inhibitor,tissue factor,lupus anticoagulan
Journal or Publication Title: Thrombosis Research
Page Range: pp. 245-249
ISSN: 0049-3848
Identification Number - DOI: 10.1016/S0049-3848(97)00124-2
Date Deposited: 10 Sep 2007
Last Modified: 18 Nov 2014 03:21
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