The investigation into the disturbance of lipid patterns in human serum by chemical agents.

The broad aim of the work reported on in this thesis was to study pathologically and chemically induced disturbances of lipid and protein patterns. Originally it was proposed to study chemically disturbed patterns by means of externally introduced lipids, especially those of dietary significance. Orientating studies on technique are given in Chapter I. The evaluation of electrophoretio lipid and protein patterns involves reference to prominent bands. It was proposed to study protein and lipoprotein patterns on cellulose acetate, which permits a complete separation of serum proteins after 1 to 1 1/2 hours electrophoresis. However, no reproducible and otherwise satisfactory method could be developed for the staining of lipids. Chapter II deals with the study of relative mobilities of serum proteins and the correlation with the work done by Parsons and Polya (1). Failure of tetracycline to affect lipaemic serum during experiments of Parsons and Polya (2) it was thought that tetracycline could be suitable as a chemical agent to bring about modification of the lipid patterns. Investigations of this hypothesis are described in Chapter III.