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Microsatellite analysis of seed production systems in Acacia mangium

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Tran, Duc Vuong (2010) Microsatellite analysis of seed production systems in Acacia mangium. Research Master thesis, University of Tasmania.

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Abstract

Acacia species, especially A. mangium, have performed well in plantations, with a
wide range of uses either local or industrial. Therefore, A. mangium has been
selected as a major plantation species in Southeast Asia. Tree improvement programs
for A. mangium have been started in several countries. The development of interspecific
hybridization techniques has been an important new-development for Acacia
species because of the outstanding performance of selected clones of the A. mangium
xA. auriculiformis hybrid in plantation forestry. In addition, in recent years,
tetraplc:>i~ clones of A. mangium have been introduced to Vietnam. It was hoped that
tetraploid A. mangium could be used to develop triploid individuals which might
have the advantage of sterility or low fertility; and in the case of triploid A. mangium
x A. auriculiformis retain the superior growth performance and/or wood properties
_ of the hybrid. This thesis reports two studies that used micro satellite markers to
support tree improvement for A. mangium.
The first study used seven microsatellite markers to test the success of different
_ methods of producing control-pollinated seed of A. mangium, with and without
emasculation. Only 3% of selfing and approximately 9% contamination with nontarget
outcross pollen were detected in un-emasculated methods, while the level of
contamination found in emasculated methods was 19%. No difference in the levels of
selfing and contamination were found between methods when a sugar solution was
added to the female flowers prior to pollination. It is suggested that emasculation
may not be necessary to produce controlled pollinated seed for A. mangium.
The second study also used seve~ microsatellite markers to compare outcrbssing
rates between diploid and tetraploid A. mangium in a clonal seed orchard that was
established in Vietnam with.alternate rows oftetraploidA. mangium, diploid A.
mangium and diploid A. auriculiformis. There was a large and 'significant difference
in outcrossing rate between diploid clones which had almost complete outcrossing _
(97%, s.e. 5.3%) and tetraploidA. mangium clones that displayed a very low degree
of outcrossing (1.9%, s.e. 1.1 %). One of the main purposes of establishment of this
mixed seed orchard was to produce triploid seeds. However, no triploid progeny
were found among 96 diploid and 161 tetraploid seedlings, while three tetraploid progeny were confirmed to be outcrosses between different teraploid clones. Several
explanations for the absence of triploids are considered. While it is probable that
there may be a barrier to pollination between diploid and tetraploid clones in this
seed orchard, phenological data showed that the flowering times of adjacent diploid
and tetraploid individuals overlapped, so the barrier to production of triploids does
not appear to be the result of differences in flowering time.

Item Type: Thesis (Research Master)
Keywords: Mangium, Microsatellites (Genetics)
Copyright Holders: The Author
Copyright Information:

Copyright 2010 the Author

Additional Information:

Available for use in the Library and copying in accordance with the Copyright Act 1968, as amended. Thesis (MSC)--University of Tasmania, 2010. Includes bibliographical references

Date Deposited: 04 Feb 2015 23:36
Last Modified: 11 Mar 2016 05:53
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