Library Open Repository

Phylogenetic and functional diversity of the cultivable bacterial community associated with the paralytic shellfish poisoning dinoflagellate Gymnodinium catenatum

Downloads

Downloads per month over past year

Green, DH and Llewellyn, LE and Negri, AP and Blackburn, SI and Bolch, CJS (2004) Phylogenetic and functional diversity of the cultivable bacterial community associated with the paralytic shellfish poisoning dinoflagellate Gymnodinium catenatum. FEMS Microbiology Ecology, 47 (3). pp. 345-357. ISSN 0168-6496

[img] PDF
fems-bolch.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.

Abstract

Gymnodinium catenatum is one of several dinoflagellates that produce a suite of neurotoxins called the paralytic shellfish toxins (PST), responsible for outbreaks of paralytic shellfish poisoning in temperate and tropical waters. Previous research suggested that the bacteria associated with the surface of the sexual resting stages (cyst) were important to the production of PST by G. catenatum. This study sought to characterise the cultivable bacterial diversity of seven different strains of G. catenatum that produce both high and abnormally low amounts of PST, with the long-term aim of understanding the role the bacterial flora has in bloom development and toxicity of this alga. Sixty-one bacterial isolates were cultured and phylogenetically identified as belonging to the Proteobacteria (70%), Bacteroidetes (26%) or Actinobacteria (3%). The Alphaproteobacteria were the most numerous both in terms of the number of isolates cultured (49%) and were also the most abundant type of bacteria in each G. catenatum culture. Two phenotypic (functional) traits inferred from the phylogenetic data were shown to be a common feature of the bacteria present in each G. catenatum culture: firstly, Alphaproteobacteria capable of aerobic anoxygenic photosynthesis, and secondly, Gammaproteobacteria capable of hydrocarbon utilisation and oligotrophic growth. In relation to reports of autonomous production of PST by dinoflagellate-associated bacteria, PST production by bacterial isolates was investigated, but none were shown to produce any PST-like toxins. Overall, this study has identified a number of emergent trends in the bacterial community of G. catenatum which are mirrored in the bacterial flora of other dinoflagellates, and that are likely to be of especial relevance to the population dynamics of natural and harmful algal blooms.

Item Type: Article
Keywords: Dinoflagellate; Paralytic shellfish poisoning; Small subunit rDNA; Cultivable bacterial diversity; Rhodobacteraceae; Alteromonadaceae; Aerobic anoxygenic photosynthesis; Hydrocarbon utilisation; Gymnodinium catenatum
Journal or Publication Title: FEMS Microbiology Ecology
Page Range: pp. 345-357
ISSN: 0168-6496
Identification Number - DOI: 10.1016/S0168-6496(03)00298-8
Additional Information: The definitive version is available at http://www.sciencedirect.com/
Date Deposited: 15 Nov 2007 06:16
Last Modified: 18 Nov 2014 03:25
URI: http://eprints.utas.edu.au/id/eprint/2477
Item Statistics: View statistics for this item

Repository Staff Only (login required)

Item Control Page Item Control Page