Library Open Repository

An electrospray mass spectrometric and voltammetric study of horse heart cytochrome c in the presence of metal ions

Downloads

Downloads per month over past year

Bond, AM and Colton, R and D'Agostino, A and Traeger, JC and Downard, AJ and Canty, AJ (1998) An electrospray mass spectrometric and voltammetric study of horse heart cytochrome c in the presence of metal ions. Inorganica Chimica Acta, 267 (2). pp. 281-291. ISSN 0020-1693

[img] PDF
ICA1998_2C_281.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.

Abstract

Studies on cytochrome c in the presence of a range of biologically essential (Ca2+, Mg2+, Zn2+, Ni2+ and Co2+ ) and toxic ( MeHg+, Cd2+ and Pb2+) metal ions have been undertaken by both cyclic voltammetry at a 4,4'-bipyridyi disulfide modified gold electrode and by electrospray mass spectrometry. Some bases for comparison of data obtained by the two techniques were achieved by compromising solvent systems and electrolyte (buffer) concentrations with which these techniques are commonly used. The most compatible solvent/electrolyte system for these techniques was found to be 65:35 water:isopropanol with acetic acid, and/or ammonium acetate being added to provide acid, electrolyte and buffer. The electrospray mass spectra of cytochrome c obtained at increased acid concentrations showed a shift in the spectral prolile to lower mass-to-charge ratios, indicating an increase in the charge density of cytochrome c. The additiion of metal salts to the electrospray solution produced metal ion adduction, the number of metal ions binding to the protein increasing with metal salt concentration. Howevcr, the adduc|ion of metal ions to cytochrome c did not alter the overall charge of the protein. Electrospray mass spectrometry and cyclic voltammetry showed that the heme group was neither lost nor replaced by any of the added metal ions, Voltammetric measurements indicate that no preferential binding of metal ions to either the Fe(Ill) or Fe(II) oxidation states ccurs, since the reversible potential obtained from voltammetric measurements did not change following tile metal salt additions.

Item Type: Article
Journal or Publication Title: Inorganica Chimica Acta
Page Range: pp. 281-291
ISSN: 0020-1693
Identification Number - DOI: 10.1016/S0020-1693(97)05775-7
Additional Information: The definitive version is available at http://www.sciencedirect.com
Date Deposited: 16 Jan 2008 00:01
Last Modified: 18 Nov 2014 03:27
URI: http://eprints.utas.edu.au/id/eprint/2881
Item Statistics: View statistics for this item

Repository Staff Only (login required)

Item Control Page Item Control Page