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Restriction fragment length polymorphisms in chloroplast DNA from six species of eucalyptus

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Steane, DA and West, AK and Potts, BM and Ovenden, JR and Reid, JB (1991) Restriction fragment length polymorphisms in chloroplast DNA from six species of eucalyptus. Australian Journal of Botany, 39 (5). pp. 399-414. ISSN 0067-1924

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Abstract

Chloroplast DNA was extracted from six species of Eucalyptus (E. perriniana, E. nitens, E. ovata, E. regnans, E. amygdalina and E. risdonii). Digests with four restriction enzymes (Hind III, Xho I, Nco I and Eco RV) revealed restriction fragment length polymorphisms (RFLPs) between subgenera, between species and within species. However, no variation in fragment pattern was detected with Sac II or Pst I. The subgenera Monocalyptus and Symphyomyrtus were clearly differentiated by their RFLP patterns where, with the exception of one outlying specimen of E. amygdalina, 45% of all polymorphic fragments were specific to one or other subgenus. While species from different subgenera and series were well differentiated, it was more difficult to differentiate species within series with the low sample sizes used. However, the average net divergence between species increased with increasing taxonomic distance between species, from 0.02% within series and 0.20% between species from different series within subgenera, to 0.99% of nucleotides per nucleotide site for species from different subgenera. Based on Eco RV digests, the eucalypt chloroplast genome was estimated at 143 kb.

Item Type: Article
Journal or Publication Title: Australian Journal of Botany
Page Range: pp. 399-414
ISSN: 0067-1924
Identification Number - DOI: 10.1071/BT9910399
Additional Information: BM Potts. Copyright 1991 CSIRO. http://www.publish.csiro.au/nid/65.htm
Date Deposited: 18 Feb 2008 11:00
Last Modified: 18 Nov 2014 03:30
URI: http://eprints.utas.edu.au/id/eprint/3235
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