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Behavior of Bacillus anthracis Strains Sterne and Ames K0610 in Sterile Raw Ground Beef

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Tamplin, ML and Phillips, R and Stewart, TA and Luchansky, JB and Kelley, LC (2008) Behavior of Bacillus anthracis Strains Sterne and Ames K0610 in Sterile Raw Ground Beef. Applied and Environment Microbiology, 74 (4). pp. 1111-1116. ISSN 0099-2240

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Abstract

The behavior of Bacillus anthracis Sterne spores in sterile raw ground beef was measured at storage temperatures of 2 to 70°C, encompassing both bacterial growth and death. B. anthracis Sterne was weakly inactivated (0.003 to 0.014 log10 CFU/h) at storage temperatures of 2 to 16°C and at temperatures greater than and equal to 45°C. Growth was observed from 17 to 44°C. At these intermediate temperatures, B. anthracis Sterne displayed growth patterns with lag, growth, and stationary phases. The lag phase duration decreased with increasing temperature and ranged from approximately 3 to 53 h. The growth rate increased with increasing temperature from 0.011 to 0.496 log10 CFU/h. Maximum population densities (MPDs) ranged from 5.9 to 7.9 log10 CFU/g. In addition, the fate of B. anthracis Ames K0610 was measured at 10, 15, 25, 30, 35, 40, and 70°C to compare its behavior with that of Sterne. There were no significant differences between the Ames and Sterne strains for both growth rate and lag time. However, the Ames strain displayed an MPD that was 1.0 to 1.6 times higher than that of the Sterne strain at 30, 35, and 40°C. Ames K0610 spores were rapidly inactivated at temperatures greater than or equal to 45°C. The inability of B. anthracis to grow between 2 and 16°C, a relatively low growth rate, and inactivation at elevated temperatures would likely reduce the risk for recommended ground-beef handling and preparation procedures.

Item Type: Article
Keywords: B. anthracis, ground beef, model
Journal or Publication Title: Applied and Environment Microbiology
Page Range: pp. 1111-1116
ISSN: 0099-2240
Identification Number - DOI: 10.1128/AEM.01292-07
Date Deposited: 24 Feb 2008 22:23
Last Modified: 18 Nov 2014 03:30
URI: http://eprints.utas.edu.au/id/eprint/3294
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