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The use of ultrasonic imaging to evaluate the effect of protozoan grazing and movement on the topography of bacterial biofilms

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Parry, J and Holmes, AK and Unwin, ME and Laybourn-Parry, J (2007) The use of ultrasonic imaging to evaluate the effect of protozoan grazing and movement on the topography of bacterial biofilms. Letters In Applied Microbiology, 45 (4). pp. 364-370. ISSN 0266-8254

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Abstract

This study evaluated the effect of protozoan movement and grazing on the topography of a dual-bacterial biofilm using both conventional light microscopy and a new ultrasonic technique.

Methods and Results: Coupons of dialysis membrane were incubated in Chalkley’s medium for 3 days at 23°C in the presence of bacteria (Pseudomonas aeruginosa and Klebsiella aerogenes) alone, or in co-culture with the flagellate Bodo designis, the ciliate Tetrahymena pyriformis or the amoeba Acanthamoeba castellanii. Amoebic presence resulted in a confluent biofilm similar to the bacteria-only biofilm while the flagellate and ciliate created more diverse biofilm topographies comprising bacterial microcolonies and cavities.

Conclusions: The four distinct biofilm topographies were successfully discerned with ultrasonic imaging and the method yielded information similar to that obtained with conventional light microscopy.

Significance and Impact of the Study: Ultrasonic imaging provides a potential way forward in the development of a portable, nondestructive technique for profiling the topography of biofilms in situ, which might aid in the future management of biofouling.

Item Type: Article
Keywords: Biofilm, microcolonies, protozoa, topography, ultrasonic.
Journal or Publication Title: Letters In Applied Microbiology
Page Range: pp. 364-370
ISSN: 0266-8254
Identification Number - DOI: 10.1111/j.1472-765X.2007.02213.x
Additional Information:

The definitive version is available at www.blackwell-synergy.com

Date Deposited: 07 Mar 2008 00:34
Last Modified: 18 Nov 2014 03:31
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