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Proteomic analysis of Myrmecia pilosula (jack jumper) ant venom

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Wiese, MD and Chataway, TK and Davies, NW and Milne, RJ and Brown, SGA and Gai, W and Heddle, RJ (2006) Proteomic analysis of Myrmecia pilosula (jack jumper) ant venom. Toxicon, 47 (2). pp. 208-217. ISSN 0041-0101

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Abstract

Ant sting allergy in Australia is predominantly due to the Myrmecia pilosula species complex. Gel separation of M. pilosula venom is necessary so that the allergenic importance of each component can be defined by western blotting. However, previous PAGE methods produced suboptimal resolution and the components of each band were not precisely defined. Venom was resolved in both non-reduced and reduced form by one-dimensional acid urea PAGE, SDS-PAGE and two-dimensional acid urea-SDS PAGE. Resolved peptides were extracted and analysed by HPLC–MS. Acid urea PAGE and acid urea-SDS PAGE proved more effective than SDS-PAGE for resolution of peptides smaller than 10 kDa. All of the major peptides previously observed in M. pilosula venom were observed in gel resolved venom. Venom was found to primarily consist of peptides with molecular weight < 10 kDa, most of which contain disulfide bridges. SDS-PAGE of non-reduced venom clearly defined six higher molecular weight proteins between 26 and 90 kDa. An 8546 Da dimer named pilosulin 5 was observed, but pilosulin 4, a peptide recently proposed to be present in venom was not. A variant of pilosulin 4 here named pilosulin 4.1a, existing as an 8198 Da dimer, was observed and has been characterised.

Item Type: Article
Keywords: jack jumper, any venom, proteomics
Journal or Publication Title: Toxicon
Page Range: pp. 208-217
ISSN: 0041-0101
Identification Number - DOI: 10.1016/j.toxicon.2005.10.018
Date Deposited: 12 Mar 2008 00:24
Last Modified: 18 Nov 2014 03:31
URI: http://eprints.utas.edu.au/id/eprint/3439
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