Library Open Repository

Fast CE for combinatorial catalysis

Downloads

Downloads per month over past year

Breadmore, MC and Hodgson, R and Kennedy, DF and Messerle, BA (2008) Fast CE for combinatorial catalysis. Electrophoresis, 29 (2). pp. 491-498. ISSN 0173-0835

[img] PDF
A1-08-04.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.

Abstract

Two solvent-modified MEKC methods were developed for the quantitative analysis of heterocyclic amines synthesised using intramolecular ring closure via catalysed hydroamination. The first method was capable of resolving six of the amines (precursors and products) with a sample-to-sample injection time of 2 min employing a 20 mM borate buffer, pH 9.2 with 20 mM SDS and 5% v/v n-butanol (n-BuOH). A second low-pH method using 20 mM phosphate buffer, 100 mm SDS, 5% v/v n-BuOH and 20% v/v iso-propanol (i-PrOH) was able to resolve an additional pair of compounds with a sample-to-sample time of 3.5 min. Application of the first method to the analysis of a sample containing catalyst as well as amines placed directly in a 96-well plate showed excellent performance, with migration time and peak height and area reproducibility being less than 0.9 and 9.6%, respectively. The quantity of conversion by catalyst was calculated to be 68 ± 7%, which was in excellent agreement with the 67 ± 5% obtained by more conventional 1H NMR experiments, with the added advantage that this method is also cheaper, quicker and more amendable to high-throughput screening of combinatorial libraries.

Item Type: Article
Keywords: Catalysis • Combinatorial screening • Fast CE • High-throughput screening • Solvent modified MEKC
Journal or Publication Title: Electrophoresis
Page Range: pp. 491-498
ISSN: 0173-0835
Identification Number - DOI: 10.1002/elps.200700220
Additional Information: Funded by: Australian Research Council; Grant Number: DP0453223, DP0556759 University of New South Wales
Date Deposited: 17 Mar 2008 00:55
Last Modified: 18 Nov 2014 03:31
URI: http://eprints.utas.edu.au/id/eprint/3457
Item Statistics: View statistics for this item

Repository Staff Only (login required)

Item Control Page Item Control Page