Peak-splitting in reversed-phase ion-pair HPLC of sympathomimetic drugs and its probable mechanism
Low, GKC and Duffield, AM and Haddad, PR (1982) Peak-splitting in reversed-phase ion-pair HPLC of sympathomimetic drugs and its probable mechanism. Chromatographia, 15 (5). pp. 289-296. ISSN 0009-5893 (Print) 1612-1112 (Online)
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Official URL: http://dx.doi.org/10.1007/BF02265679
In the determination of ephedrine using reversed-phase, ion-pair liquid chromatography, a chromatographically pure sample was observed to give three peaks under certain mobile phase conditions. The mobile phases which produced maximum peak splitting were determined for ephedrine and a number of other sympathomimetic drugs.
A proposal that peak splitting was the result of the composite interplay of two discrete chromatographic mechanisms, was investigated. The results of analysis by GC/MS confirmed that each peak was due to ephedrine, however, only one of the three split peaks was found to contain ion pairs. It is postulated that peak splitting is a physical phenomenon on reversed-phase columns and the separation of these drugs by ion-pair HPLC is based on a mixed rather than a single mechanism.
This study has also shown that errors can arise in ion-pair HPLC when multiple peaks are assumed to indicate heterogeneity. Interconvertible species of the same solute can give rise to these peaks.
|Additional Information:||The original publication is available at http://www.springerlink.com/|
|Keywords:||Ion-pair liquid chromatography - Peak splitting - Sympathominetic drugs|
|Deposited By:||Mr Marcus Guijt|
|Deposited On:||24 Mar 2008 21:16|
|Last Modified:||16 Mar 2010 11:20|
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