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Peak-splitting in reversed-phase ion-pair HPLC of sympathomimetic drugs and its probable mechanism
journal contribution
posted on 2023-05-26, 11:27 authored by Low, GKC, Duffield, AM, Paul HaddadPaul HaddadIn the determination of ephedrine using reversed-phase, ion-pair liquid chromatography, a chromatographically pure sample was observed to give three peaks under certain mobile phase conditions. The mobile phases which produced maximum peak splitting were determined for ephedrine and a number of other sympathomimetic drugs. A proposal that peak splitting was the result of the composite interplay of two discrete chromatographic mechanisms, was investigated. The results of analysis by GC/MS confirmed that each peak was due to ephedrine, however, only one of the three split peaks was found to contain ion pairs. It is postulated that peak splitting is a physical phenomenon on reversed-phase columns and the separation of these drugs by ion-pair HPLC is based on a mixed rather than a single mechanism. This study has also shown that errors can arise in ion-pair HPLC when multiple peaks are assumed to indicate heterogeneity. Interconvertible species of the same solute can give rise to these peaks.
History
Publication title
ChromatographiaVolume
15Article number
5Number
5Pagination
289-296ISSN
0009-5893 (Prit) 1612-1112 (Olie)Publication status
- Published
Rights statement
The original publication is available at http://www.springerlink.com/Repository Status
- Restricted
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