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A DArT platform for quantitative bulked segregant analysis

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Wenzl, P and Raman, H and Wang, J and Zhou, M and Huttner, E and Kilian, A (2007) A DArT platform for quantitative bulked segregant analysis. BMC Genomics, 8. p. 196. ISSN 1471-2164

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Abstract

Bulked segregant analysis (BSA) identifies molecular markers associated with a
phenotype by screening two DNA pools of phenotypically distinct plants for markers with skewed
allele frequencies. In contrast to gel-based markers, hybridization-based markers such as SFP, DArT
or SNP generate quantitative allele-frequency estimates. Only DArT, however, combines this
advantage with low development and assay costs and the ability to be deployed for any plant species
irrespective of its ploidy level. Here we investigate the suitability of DArT for BSA applications using
a barley array as an example.

Item Type: Article
Journal or Publication Title: BMC Genomics
Publisher: BioMed Central Ltd
Page Range: p. 196
ISSN: 1471-2164
Identification Number - DOI: 10.1186/1471-2164-8-196
Additional Information:

© 2007 Wenzl et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Date Deposited: 07 Apr 2008 14:02
Last Modified: 18 Nov 2014 03:32
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