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Oxidative stress and lipid-derived inflammatory mediators during acute exacerbations of cystic fibrosis

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Reid, DW and Misso, N and Aggarwal, S and Thompson, PJ and Walters, EH (2007) Oxidative stress and lipid-derived inflammatory mediators during acute exacerbations of cystic fibrosis. Respirology, 12 (1). pp. 63-69. ISSN 1323-7799

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Abstract

Oxidative stress and lipid-derived inflammatory mediators during acute exacerbations of cystic
fibrosis
REID DW, MISSO N, AGGARWAL S, THOMPSON PJ, WALTERS EH. Respirology 2007; 12: 63–69
Background and objective: In cystic fibrosis (CF) very few studies have assessed sputum 8-iso-
PGF2α levels during pulmonary exacerbations as a direct measure of airway oxidative stress. The role
of other lipid-derived inflammatory mediators, such as the cysteinyl leukotrienes (cys-LTs) and prostaglandin
(PG)-E2, during exacerbations is also poorly defined and the effect of conventional antibiotic
therapy on these components of the inflammatory process is unclear.
Methods: Sputum 8-iso-PGF2α, total cys-LT and PGE2 levels were measured in 17 CF patients experiencing
a pulmonary exacerbation and repeated analysis were performed in 15 of these patients
after antibiotic treatment. Eight stable CF and nine healthy subjects provided control data.
Results: Sputum 8-iso-PGF2α was significantly elevated in acute, but not stable CF patients versus
healthy controls (P < 0.001). Similarly, sputum cys-LT and PGE2 levels were increased in acute compared
with stable CF patients and healthy controls (P ≤ 0.001). Although substantially lower than in
acute patients, sputum cys-LT levels in stable patients were also significantly higher than in normal
controls (P = 0.01). There were strong associations between cys-LT levels and sputum total cell
counts, and blood neutrophils in acute patients (r2 = 0.53, P = 0.001 and r2 = 0.33, P < 0.05, respectively).
Overall in the CF patients, FEV1%predicted was strongly and negatively correlated with sputum
8-iso-PGF2α (r2 = 0.34, P = 0.006), cys-LT (r2 = 0.40, P = 0.002) and PGE2 (r2 = 0.52, P < 0.001) levels.
Antibiotic treatment reduced sputum total cell count (P = 0.03), but did not affect 8-iso-PGF2α, cys-LT
or PGE2 levels.
Conclusions: CF exacerbations are characterized by increased oxidative stress and sputum concentrations
of bioactive lipid mediators. Treatment does not modulate these aspects of inflammation
and more targeted therapy needs further study.

Item Type: Article
Journal or Publication Title: Respirology
Publisher: Wiley-Blackwell Publishing Asia
Page Range: pp. 63-69
ISSN: 1323-7799
Identification Number - DOI: 10.1111/j.1440-1843.2006.00962.x
Additional Information:

The original publication is available at
http://www.interscience.wiley.com/

Date Deposited: 07 Apr 2008 14:15
Last Modified: 18 Nov 2014 03:33
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