Please Note:

The Library Open Repository will be moving to a new authentication system on the 1st of November.

From this date onwards, account holders will be required to login using their University of Tasmania credentials.
If your current repository username differs from your University username, please email E.Prints@utas.edu.au so we can update these details on your behalf.

Due to the change, there will be a short outage of the repository from 9am on the morning of the 1st of November

Library Open Repository

Transcriptome analysis reveals season-specific rbcS gene expression profiles in diploid perennial ryegrass (Lolium perenne L.)

Downloads

Downloads per month over past year

Sathish, P and Phillips, JR and Withana, N and Biswas, M and Bryant, C and Templeton, K and Al-Wahb, M and Smith-Espinoza, C and Roche, JR and Eldboroug, KM (2007) Transcriptome analysis reveals season-specific rbcS gene expression profiles in diploid perennial ryegrass (Lolium perenne L.). Plant Biotechnology Journal, 5 (1). pp. 146-161. ISSN 1467-7644

[img] PDF
3975.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.

Abstract

Perennial ryegrass (Lolium perenne L.) is a major grass species used for forage and turf throughout the world, and gains by conventional breeding have reached a plateau. Perennial ryegrass is an outcrossing, self-incompatible diploid (2n = 2x = 14) with a relatively large genome (4067 Mbp/diploid genome; Evans, G.M., Rees, H., Snell, C.L. and Sun, S. (1972) The relation between nuclear DNA amount and the duration of the mitotic cycle. Chrom. Today, 3, 24–31). Using tissues sourced from active pastures during the peak of the autumn, winter, spring and summer seasons, we analysed the ryegrass transcriptome employing a Serial Analysis of Gene Expression (SAGE™) protocol, with the dual goals of understanding the seasonal changes in perennial ryegrass gene expression and enhancing our ability to select genes for genetic manipulation. A total of 159 002 14-mer SAGE™ tags was sequenced and mapped to the perennial ryegrass DNA database, comprising methyl-filtered (GeneThresher®) and expressed sequence tag (EST) sequences. The analysis of 14 559 unique SAGE™ tags, which were present more than once in our SAGE™ library, revealed 964, 1331, 346 and 131 exclusive transcripts to autumn, winter, spring and summer, respectively. Intriguingly, our analysis of the SAGE™ tags revealed season-specific expression profiles for the small subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco), LprbcS. The transcript level for LprbcS was highest in spring, and then decreased gradually between summer and winter. Five different copies of LprbcS were revealed in ryegrass, with one possibly producing splice variant transcripts. Two highly expressed LprbcS genes were reported, one of which was not active in autumn. Another LprbcS gene showed an inverse expression profile to the autumn inactive LprbcS in a manner to compensate the expression level.

Item Type: Article
Keywords: paddock samples • pasture • perennial ryegrass • rbcS • Serial Analysis of Gene Expression (SAGE™) • season-specific expression
Journal or Publication Title: Plant Biotechnology Journal
Publisher: Blackwell Publishing Ltd
Page Range: pp. 146-161
ISSN: 1467-7644
Identification Number - DOI: 10.1111/j.1467-7652.2006.00228.x
Additional Information:

The definitive published version is available online at: http://interscience.wiley.com

Date Deposited: 07 Apr 2008 14:15
Last Modified: 18 Nov 2014 03:33
Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page
TOP