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Automation of solid-phase microextraction on a 96-well plate format

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Hutchinson, JP and Setkova, L and Pawliszyn, J (2007) Automation of solid-phase microextraction on a 96-well plate format. Journal of Chromatography A, 1149 (2). pp. 127-137. ISSN 0021-9673

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Abstract

Studies have been performed assessing the feasibility and characterizing the automation of solid-phase microextraction (SPME) on a multi-well plate format. Four polycyclic aromatic hydrocarbons (PAHs), naphthalene, fluorene, anthracene and fluoranthene, were chosen as test analytes to demonstrate the technique due to their favorable partition coefficients, Kfw, between polydimethylsiloxane (PDMS) extraction phases and water. Four different PDMS configurations were investigated regarding their suitability. These included (i) a PDMS membrane; (ii) a multi-fiber device containing lengths of PDMS-coated flexible wire; (iii) a stainless steel pin covered with silicone hollow fiber membrane and (iv) commercial PDMS-coated flexible metal fiber assemblies. Of these configurations, the stainless steel pin covered with silicone tubing was chosen as a robust alternative. An array of 96 SPME devices that can be placed simultaneously into a 96-well plate was constructed to demonstrate the high-throughput potential when performing multiple microextractions in parallel. Different agitation methods were assessed including magnetic stirring, sonication, and orbital shaking at different speeds. Orbital shaking whilst holding the SPME device in a stationary position provided the optimum agitation conditions for liquid SPME. Once the analytes had been extracted, desorption of the analytes into an appropriate solvent was investigated. Liquid-phase SPME and solvent desorption on the multi-well plate format is shown to be a viable alternative for automated high-throughput SPME analysis compatible with both gas- and liquid-chromatography platforms.

Item Type: Article
Keywords: Automation; Solid-phase microextraction; 96-well plate; Multi-well plate; PAH; polydimethylsiloxane; Membrane; Multi-fiber
Journal or Publication Title: Journal of Chromatography A
Publisher: Elsevier Science
Page Range: pp. 127-137
ISSN: 0021-9673
Identification Number - DOI: 10.1016/j.chroma.2007.02.117
Additional Information:

The definitive version is available at http://www.sciencedirect.com

Date Deposited: 07 Apr 2008 14:18
Last Modified: 18 Nov 2014 03:34
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