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Elevated plasma glutathione peroxidase concentration in acute severe asthma: Comparison with plasma glutathione peroxidase activity, selenium and malondialdehyde

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Jacobson, GA and Yee, KC and Ng, CH (2007) Elevated plasma glutathione peroxidase concentration in acute severe asthma: Comparison with plasma glutathione peroxidase activity, selenium and malondialdehyde. Scandinavian Journal of Clinical and Laboratory Investigation, 67 (4). pp. 423-430. ISSN 0036-5513

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Abstract

To investigate plasma glutathione peroxidase concentration, glutathione peroxidase
activity, plasma selenium and oxidative stress in acute severe asthma. Material and methods. The
study was case-control in design, with cases presenting to the emergency department with acute
severe asthma and controls randomly selected from a larger cross-sectional study. Plasma
malondialdehyde (MDA) was used as a measure of oxidative stress and plasma selenium was
measured using ICP-MS. Glutathione peroxidase (GPx) activity was analysed using a colorimetric
GPx assay and plasma GPx level was measured by enzyme-linked immunosorbent assay (ELISA).
Results. Fifteen cases [mean (range) predicted peak expiratory flow rate (PEFR) of 43% (20–69)] and
15 matched controls were recruited. MDA levels (mean¡SD) were higher in acute asthma subjects
(1.30¡0.56 mmol/L) than in controls (0.86¡0.53 mmol/L; pv0.05). There were no differences
between cases and controls for selenium (99¡34 mg/L versus 109¡17 mg/L) or for GPx activity
(39¡25 nmol min21 mL21 versus 38¡24 nmol min21 mL21), however, GPx plasma levels measured
by ELISA were higher in cases than controls (22.5¡10.8 mg/L versus 13.8¡7.3 mg/L; pv0.05).
Conclusions. Patients with acute severe asthma demonstrated increasedMDA levels but no differences
in plasma selenium levels or GPx activity. GPx levels measured by ELISA were elevated in severe
asthma. These results are consistent with an adaptive up-regulation of GPx to protect against oxidative
stress.

Item Type: Article
Keywords: Antioxidant, emergency medicine, enzymes, oxidative stress, selenoprotein
Journal or Publication Title: Scandinavian Journal of Clinical and Laboratory Investigation
Publisher: Informa Healthcare
Page Range: pp. 423-430
ISSN: 0036-5513
Identification Number - DOI: 10.1080/00365510601153353
Additional Information:

The definitive version is available online at
http://www.informaworld.com/smpp/content?

Date Deposited: 07 Apr 2008 14:30
Last Modified: 18 Nov 2014 03:35
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