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Theoretical migration model for micellar capillary electrophoresis and its application to the separation of anionic metal complexes of HEDTC and CDTA

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Breadmore, MC and Macka, M and Haddad, PR (1999) Theoretical migration model for micellar capillary electrophoresis and its application to the separation of anionic metal complexes of HEDTC and CDTA. Analytical Chemistry, 71 (9). pp. 1826-1833. ISSN 0003-2700

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Abstract

A mathematical model relating the effective mobility of an analyte in micellar capillary electrophoresis (MCE) to the concentration of surfactant and organic modifier in the background electrolyte (BGE) was derived. Effective mobility is expressed in terms of the electrophoretic mobility of the analyte, the partition coefficient of the analyte into the micelle, and the influence of organic modifier on these two factors. The performance of the model was evaluated using Cd(II), Pb(II), Co(II), Ni(II), Bi(III), Cu(II), and Hg(II) complexes of bis(2-hydroxyethyl)dithiocarbamate, all of which carry a partial negative charge, and Cd(II), Pb(II), Co(II), Ni(II), Bi(III), Cu(II), Hg(II), Fe(III), Ag(I), Tl(I), and Mn(II) complexes of trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid, all of which are anionic having charges in the range -1 to -3. These analytes were separated in borate BGEs containing 10-50 mM sodium dodecyl sulfate and 0-20% (v/v) methanol. Nonlinear regression was used to derive parameters for the model from experimental data and these parameters were used to predict effective mobilities of the analytes. Predicted values of effective mobilties agreed with experimental values to within 3.1%. Values of parameters from the model equation are used to explain changes in separation selectivity observed at different BGE compositions and the model equation is shown to be applicable to computer-assisted optimization of the BGE composition, in MCE using a limited number of experiments.

Item Type: Article
Journal or Publication Title: Analytical Chemistry
Page Range: pp. 1826-1833
ISSN: 0003-2700
Identification Number - DOI: 10.1021/ac981044h
Additional Information: Copyright © 1999 American Chemical Society
Date Deposited: 13 May 2008 23:31
Last Modified: 18 Nov 2014 03:40
URI: http://eprints.utas.edu.au/id/eprint/6337
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