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A molecular approach to identify prey of the southern rock lobster
Redd, KS and Jarman, SN and Frusher, SD and Johnson, CR (2008) A molecular approach to identify prey of the southern rock lobster. Bulletin of Entomological Research, 98 (3). pp. 233-238. ISSN 0007-4853
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We demonstrate the use of molecular techniques to detect specific prey consumed by the southern rock lobster ( Jasus edwardsii). A quick and non-lethal method was used to collect rock lobster faecal material and a molecular protocol was employed to isolate prey DNA from faecal samples. The isolated DNA was amplified using the polymerase chain reaction (PCR) with PCR primers designed to target specific prey items. Feeding experiments determined that DNA from black-lipped abalone (Haliotis rubra) and sea urchins (Centrostephanus rodgersii and Heliocidaris erythrogramma) can be detected in rock lobster faecal samples within seven hours and remains present for up to 60 h after ingestion.
|Keywords:||Jasus edwardsii, Haliotis rubra, polymerase chain reaction (PCR), prey detection, rock lobster diet|
|Journal or Publication Title:||Bulletin of Entomological Research|
|Page Range:||pp. 233-238|
|Identification Number - DOI:||10.1017/S0007485308005981|
|Date Deposited:||25 May 2008 23:55|
|Last Modified:||18 Nov 2014 03:41|
|Item Statistics:||View statistics for this item|
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