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Sensitive determination of carbohydrates labelled with p-nitroaniline by capillary electrophoresis with photometric detection using a 406 nm light-emitting diode

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Momenbeik, F and Johns, CA and Breadmore, MC and Hilder, EF and Macka, M and Haddad, PR (2006) Sensitive determination of carbohydrates labelled with p-nitroaniline by capillary electrophoresis with photometric detection using a 406 nm light-emitting diode. Electrophoresis, 27 (20). pp. 4039-4046. ISSN 0173-0835

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Abstract

p-Nitroaniline was explored as a derivatising reagent for UV absorbance detection of carbohydrates after separation by CE. This derivatising agent has three advantages: first, it has excellent water solubility; second, it has high molar absorptivity; and third, it is possible to obtain sensitive detection using a UV or blue light-emitting diode (LED) as the light source. The labelling reaction took less than 30 min to complete with high reaction yield. The separation process was modelled and optimised using an artificial neural network. Nine carbohydrates were separated by a CE system within 16 min using a 0.17 M boric acid buffer at pH 9.7. On-column LED detection at 406 nm allowed the detection of carbohydrates with good detection limits (<1.1 µM or 8.8 fmol) and reproducible quantification in the concentration range of 2.6-200 µM. This method was applied successfully to the determination of component carbohydrates in some food samples.

Item Type: Article
Keywords: Blue light-emitting diode • Capillary electrophoresis • Carbohydrates • Derivatisation • p-Nitroaniline
Journal or Publication Title: Electrophoresis
Page Range: pp. 4039-4046
ISSN: 0173-0835
Identification Number - DOI: 10.1002/elps.200500856
Additional Information: http://www3.interscience.wiley.com/journal/113307121/abstract
Date Deposited: 03 Jun 2008 12:22
Last Modified: 18 Nov 2014 03:41
URI: http://eprints.utas.edu.au/id/eprint/6588
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