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Determination of ribavirin in human serum and plasma by capillary electrophoresis

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Breadmore, MC and Theurillat, R and Thormann, W (2004) Determination of ribavirin in human serum and plasma by capillary electrophoresis. Electrophoresis, 25 (10-11). pp. 1615-1622. ISSN 0173-0835

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Abstract

The electrophoretic separation of ribavirin and 5-methylcytidine (internal standard) by capillary electrophoresis was examined. Separation was achieved using reverse polarity in a 100 mM borate electrolyte, pH 9.1, with 5 mM spermine added to reduce the electroosmotic flow. Sample preparation based on acetonitrile protein precipitation was found to be unsuitable for ribavirin analysis in patient samples due to insufficient sensitivity and interferences. Solid-phase extraction employing phenyl boronic acid cartridges provided cleaner separations. Using this approach with 500 {micro}L sample and reconstitution of the dried extract into 100 {micro}L of 33% v/v 100 mM phosphate buffer, pH 6.4 / 67% v/v acetonitrile, the detection and quantitation limits were determined to be 0.05 and 0.10 {micro}g/mL, respectively, a sensitivity that is suitable for therapeutic drug monitoring of ribavirin in human plasma and serum samples. The method was validated and compared to a high-performance liquid chromatography (HPLC) method, showing excellent agreement between the two for a set of samples that stemmed from patients being treated with ribavirin and interferon-{alpha}-2b for a hepatitis C virus infection.

Item Type: Article
Keywords: Capillary electrophoresis • High-performance liquid chromatography • Ribavirin • Therapeutic drug monitoring
Journal or Publication Title: Electrophoresis
Page Range: pp. 1615-1622
ISSN: 0173-0835
Identification Number - DOI: 10.1002/elps.200305819
Additional Information: Copyright © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Date Deposited: 11 Jun 2008 12:05
Last Modified: 18 Nov 2014 03:42
URI: http://eprints.utas.edu.au/id/eprint/6644
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