Library Open Repository

Determination of itraconazole and hydroxyitraconazole in human serum and plasma by micellar electrokinetic chromatography

Downloads

Downloads per month over past year

Breadmore, MC and Prochazkova, A and Theurillat, R and Thormann, W (2003) Determination of itraconazole and hydroxyitraconazole in human serum and plasma by micellar electrokinetic chromatography. Journal of Chromatography A, 1014 (1-2). pp. 57-70. ISSN 0021-9673

[img] PDF
024.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.

Abstract

The electrokinetic separation of the hydrophobic antimycotic drug itraconazole (ITC) and its major metabolite, hydroxyitraconazole (HITC), by a binary aqueous–organic solvent medium containing sodium dodecylsulfate, by microemulsion electrokinetic chromatography (MEEKC) and by micellar electrokinetic chromatography (MEKC) was studied. The results suggest that the first approach is difficult to apply and that there is no substantial difference between separations performed using MEEKC and MEKC modified with n-butanol. The simpler MEKC method is more than adequate and was thus employed for the analysis of ITC and HITC in human serum and plasma. Separation was achieved in plain fused-silica capillaries having a low-pH buffer (pH 2.2) with sodium dodecyl sulfate micelles and reversed polarity. The addition of 2-propanol and n-butanol enhanced analyte solubility and altered the selectivity of the separation by influencing the magnitude of the electrophoretic component in the separation mechanism. Under optimised conditions and using head-column field-amplified sample stacking, an internal standard, ITC and two forms of HITC could be separated in under 9 min, with detection limits less than 0.01 μg/mL. Analysis of samples from patients currently prescribed ITC revealed a different HITC peak area ratio to that of the standards, suggesting a stereoselective component of ITC metabolisation. Comparison of MEKC data with those of a HPLC method employed on a routine basis showed excellent agreement, indicating the potential of this approach for therapeutic drug monitoring of ITC.

Item Type: Article
Keywords: Itraconazole; Hydroxyitraconazole; Microemulsion electrokinetic chromatography; Electrokinetic chromatography
Journal or Publication Title: Journal of Chromatography A
Page Range: pp. 57-70
ISSN: 0021-9673
Identification Number - DOI: 10.1016/S0021-9673(03)00573-9
Additional Information: Copyright © 2003 Elsevier B.V. All rights reserved. 16th International Symposium on Microscale Separations and Analysis. Part II
Date Deposited: 11 Jun 2008 12:15
Last Modified: 18 Nov 2014 03:42
URI: http://eprints.utas.edu.au/id/eprint/6652
Item Statistics: View statistics for this item

Repository Staff Only (login required)

Item Control Page Item Control Page