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Expression of natriuretic peptide receptor mRNA and functional response to atrial natriuretic peptide and C-type natriuretic peptide in rainbow trout (Oncorhynchus mykiss) head kidney leucocytes

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Powell, MD and McWilliam, H and McLeod, J and Nankervis, S and Butler, R and Toop, T (2008) Expression of natriuretic peptide receptor mRNA and functional response to atrial natriuretic peptide and C-type natriuretic peptide in rainbow trout (Oncorhynchus mykiss) head kidney leucocytes. Fish and Shellfish Immunology, 24 (4). pp. 373-378. ISSN 1050-4648

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Abstract

Abstract The stimulatory effect of vasomodulatory natriuretic peptide hormones on macrophages and peripheral blood leucocytes in mammals is well-established. However, the relationship in lower vertebrates has not been characterised. Expression of atrial natriuretic peptide, ventricular natriuretic peptide and C-type natriuretic peptide-1, and the guanylyl cyclaselinked (GC) natriuretic peptide receptor-A and -B-type receptors (NPR-A and NPR-B, respectively) was determined by PCR from the mRNA of rainbow trout head kidney leucocytes yielding gene fragments with 100% homology to the same respective natriuretic peptide and NPR-A and -B sequences obtained from other rainbow trout tissues. A mixed population of isolated rainbow trout head kidney leucocytes was stimulated in vitro with trout atrial natriuretic peptide (specific NPR-A agonist) and trout C-type natriuretic peptide (NPR-A and -B agonist) as well as the cGMP agonist 8-bromo-cGMP or the GC inhibitor 8-bromo-phenyl-eutheno-cGMP. Respiratory burst was stimulated by trout atrial natriuretic peptide, trout C-type natriuretic peptide-1 and 8-bromo-cGMP in a dose dependant manner with the highest activity as a result of stimulation with trout C-type natriuretic peptide-1 in excess of that achieved by phorbol myristate acetate (PMA). Equimolar concentrations of the inhibitor, inhibited the respiratory burst caused by the natriuretic peptides and 8-bromo-cGMP. The natriuretic peptide receptors on rainbow

Item Type: Article
Keywords: Natriuretic peptides; Leukocytes; Rainbow trout; Respiratory burst
Journal or Publication Title: Fish and Shellfish Immunology
Page Range: pp. 373-378
ISSN: 1050-4648
Identification Number - DOI: 10.1016/j.fsi.2007.08.003
Additional Information: The definitive version is available at http://www.sciencedirect.com
Date Deposited: 11 Jun 2008 00:48
Last Modified: 18 Nov 2014 03:42
URI: http://eprints.utas.edu.au/id/eprint/6752
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