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Development of a diagnostic PCR to detect Neoparamoeba perurans, agent of amoebic gill disease

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Young, ND and Dykova, I and Nowak, BF and Morrison, RN (2008) Development of a diagnostic PCR to detect Neoparamoeba perurans, agent of amoebic gill disease. Journal of Fish Diseases, 31 (4). pp. 285-295. ISSN 0140-7775

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Abstract

Abstract
The recent description of Neoparamoeba perurans as
an aetiological agent of amoebic gill disease (AGD)
advanced our understanding of the condition and
has forced a re-evaluation of methods used for the
diagnosis of AGD. Currently, there are no tools
available that are both specific for N. perurans and
suitable for a routine diagnostic procedure. Therefore,
in this study we describe an assay to detect
N. perurans. The assay, which utilizes PCR to
amplify the N. perurans 18S rRNA gene, was
shown to be specific and highly sensitive. Neoparamoeba
perurans was detected in both gill samples
and primary isolates of non-cultured gill-derived
amoebae obtained during necropsy or biopsy from
AGD-affected Atlantic salmon, Salmo salar L. The
PCR-based assay provides a simple, flexible tool
that will be a useful addition to the diagnostic
repertoire for AGD. It may also be used for the
genotypic screening of trophozoites during culture
and could facilitate further epidemiological and
ecological studies of AGD.
Keywords: amoebic gill disease, detection, Neoparamoeba
perurans, polymerase chain reaction.

Item Type: Article
Keywords: amoebic gill disease, detection, Neoparamoeba perurans, polymerase chain reaction
Journal or Publication Title: Journal of Fish Diseases
Page Range: pp. 285-295
ISSN: 0140-7775
Identification Number - DOI: 10.1111/j.1365-2761.2008.00903.x
Additional Information:

The definitive version is available at www.blackwell-synergy.com

Date Deposited: 12 Jun 2008 04:52
Last Modified: 18 Nov 2014 03:42
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