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Microchip-based purification of DNA from biological samples

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Breadmore, MC and Wolfe, KA and Arcibal, IG and Leung, WK and Dickson, D and Giordano, BC and Power, ME and Ferrance, JP and Feldman, SH and Norris, PM and Landers, JP (2003) Microchip-based purification of DNA from biological samples. Analytical Chemistry, 75 (8). pp. 1880-1886. ISSN 0003-2700

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Abstract

A microchip solid-phase extraction method for purification of DNA from biological samples, such as blood, is demonstrated. Silica beads were packed into glass microchips and the beads immobilized with sol-gel to provide a stable and reproducible solid phase onto which DNA could be adsorbed. Optimization of the DNA loading conditions established a higher DNA recovery at pH 6.1 than 7.6. This lower pH also allowed for the flow rate to be increased, resulting in a decrease in extraction time from 25 min to less than 15 min. Using this procedure, template genomic DNA from human whole blood was purified on the microchip platform with the only sample preparation being mixing of the blood with load buffer prior to loading on the microchip device. Comparison between the microchip SPE ({micro}chipSPE) procedure and a commercial microcentrifuge method showed comparable amounts of PCR-amplifiable DNA could be isolated from cultures of Salmonella typhimurium. The greatest potential of the {micro}chipSPE device was illustrated by purifying DNA from spores from the vaccine strain of Bacillus anthracis, where eventual integration of SPE, PCR, and separation on a single microdevice could potentially enable complete detection of the infectious agent in less than 30 min.

Item Type: Article
Journal or Publication Title: Analytical Chemistry
Page Range: pp. 1880-1886
ISSN: 0003-2700
Identification Number - DOI: 10.1021/ac0204855
Additional Information: Copyright © 2003 American Chemical Society.
Date Deposited: 23 Jun 2008 01:01
Last Modified: 18 Nov 2014 03:42
URI: http://eprints.utas.edu.au/id/eprint/6779
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