Please Note:

The Library Open Repository will be moving to a new authentication system on the 1st of November.

From this date onwards, account holders will be required to login using their University of Tasmania credentials.
If your current repository username differs from your University username, please email so we can update these details on your behalf.

Due to the change, there will be a short outage of the repository from 9am on the morning of the 1st of November

Library Open Repository

Towards a microchip-based chromatographic platform. Part 2: Sol-gel phases modified with polyelectrolyte multilayers for capillary electrochromatography


Downloads per month over past year

Breadmore, MC and Shrinivasan, S and Ferrance, JP and Power, ME and Norris, PM and Landers, JP and Karlinsey, JM (2003) Towards a microchip-based chromatographic platform. Part 2: Sol-gel phases modified with polyelectrolyte multilayers for capillary electrochromatography. Electrophoresis, 24 (7-8). pp. 1261-1270. ISSN 0173-0835

[img] PDF
022.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.


The potential for using polyelectrolyte multilayers (PEMs) to provide chromatographic functionality on continuous silica networks created from sol-gel chemistry has been evaluated by capillary electrochromatography (CEC). Construction of the PEM was achieved by flushing the column with polyelectrolytes of alternative charge, with variation of the properties of the exposed polyelectrolyte providing a unique means to vary the chromatographic surface. Variation of the exposed polyelectrolyte from poly(diallyldimethylammonium chloride) (PDDAC) to dextran sulfate (DS) allowed the direction of the electroosmotic flow (EOF) to be changed and also provided a means to vary the chromatographic capacity. Variation of negative polymer from DS to poly(styrene sulfonate) (PSS) significantly altered the EOF and the migration of peptides, with both the reversed-phase and ion-exchange capacities increasing. An alternative method for changing the column capacity was to change the thickness of the PEM, which was evaluated by anion-exchange CEC. A 70-80% increase in retention was observed for all anions without any increase in EOF suggesting significant penetration of the analytes through the PEM and interaction with buried charges within the PEM.

Item Type: Article
Keywords: Capillary electrochromatography • Miniaturization • Polyelectrolyte multilayers • Silica monolithic column • Sol-gel phase
Journal or Publication Title: Electrophoresis
Page Range: pp. 1261-1270
ISSN: 0173-0835
Identification Number - DOI: 10.1002/elps.200390162
Additional Information:

The original publication is available at

Date Deposited: 12 Jun 2008 05:16
Last Modified: 18 Nov 2014 03:42
Item Statistics: View statistics for this item

Actions (login required)

Item Control Page Item Control Page