Monolithic sol-gel microchip device for efficient isolation of nucleic acid from clinical samples

Wu, Qirong and Breadmore, MC and Hassan, BJ and Giordano, BC and Kwok, YC and Karlinsey, JM and Ferrance, JP and Shrinivasan, S and Norris, PM and Landers, JP (2002) Monolithic sol-gel microchip device for efficient isolation of nucleic acid from clinical samples. In: Micro Total Analysis Systems 2002, 3-7 November, 2002, Nara, Japan.

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Official URL: http://www.springer.com/chemistry/physical/book/978-1-4020-1011-8

Abstract

The feasibility of using monolithic sol-gels for micro-solid phase extraction ({micro}SPE) of deoxyribonucleic acid (DNA) in a microchip format is explored in this work. The monolithic sol-gel bed in a microchip allows efficient DNA purification and extraction with back pressures that are significantly lower than a particle-packed extraction bed. The applicability of the developed {micro}SPE DNA extraction protocol to clinical samples, for example blood and serum, is successfully demonstrated.

Item Type:	Conference or Workshop Item (UNSPECIFIED)
ID Code:	6891
Deposited By:	Mr Marcus Guijt
Deposited On:	06 Jul 2008 20:16
Last Modified:	18 Jul 2008 21:06
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