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On-line concentration of neutral analytes for micellar electrokinetic chromatography IV. Field-enhanced sample injection


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Quirino, JP and Terabe, S (1998) On-line concentration of neutral analytes for micellar electrokinetic chromatography IV. Field-enhanced sample injection. Journal of Chromatography A, 798 (1-2). pp. 251-257. ISSN 0021-9673

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Fundamental conditions for the on-line concentration of neutral analytes by field-enhanced sample injection (FESI) for micellar electrokinetic chromatography (MEKC) is presented. Long water plugs are injected into the capillary to effect an enhanced field during electrokinetic injection of samples at negative polarity. Optimization of the injection procedure is performed by modifying the composition of the micellar sample matrix containing one anionic surfactant. Stacking is found to be dependent on analyte retention factors and the nature of the pseudostationary phase. More than 20- and 100-fold improvements in peak height and peak area were confirmed experimentally. The applicability of the technique was tested using spiked urine samples following a simple liquid–liquid extraction step.

Item Type: Article
Keywords: Resorcinol; Naphthols; Dihydroxynaphthalene; Estrogens; Steroids; Injection methods; Micellar electrokinetic chromatography; Sample stacking; Sample handling; Field-enhanced sample injection; Electrokinetic injection
Journal or Publication Title: Journal of Chromatography A
Page Range: pp. 251-257
ISSN: 0021-9673
Identification Number - DOI: 10.1016/S0021-9673(97)01209-0
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Date Deposited: 30 Jun 2008 22:50
Last Modified: 18 Nov 2014 03:44
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