Sorbents with immobilized glycopeptide antibiotics for separating optical isomers by high-performance liquid chromatography

Abstract

Chiral sorbents for HPLC separation of optical isomers carrying glycopeptide antibiotics (eremomycin or its eremosaminyl aglycon, ristomycin, or vancomycin) fixed onto the surface of silica gel have been synthesized. The patterns of the retention and separation of profen isomers and their dependence on the nature of the chiral selector and the eluant composition have been studied. The sorbents were shown to be highly enantiospecific in separating the isomers of α-amino-, α-hydroxy-, and α-methylphenylcarboxylic acids (profens)