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Separation of inorganic anions on a high capacity porous polymeric monolithic column and application to direct determination of anions in seawater

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Evenhuis, CJ and Buchberger, W and Hilder, EF and Flook, KJ and Pohl, CA and Nesterenko, PN and Haddad, PR (2008) Separation of inorganic anions on a high capacity porous polymeric monolithic column and application to direct determination of anions in seawater. Journal of Separation Science, 31 (14). pp. 2598-2604. ISSN 1615-9306

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Abstract

A commercially available 4.6 mm id×50 mm polymethacrylate-based monolithic strong anion exchange column (ProSwiftTM SAX-1S) designed for the separation of proteins has been successfully used to separate small inorganic anions in the presence of a seawater sample matrix. Using a hydroxide eluent with suppressed conductivity detection the ion exchange capacity of this column declined over time; however, using KCl as the eluent, the column performance was stable with a capacity of 530 {micro}equiv. for nitrate. The optimum conditions for the separation of iodate, bromate, nitrite, bromide and nitrate were assessed by constructing van Deemter plots using 1.00 and 0.100 M KCl. Efficiencies of up to 26 700 plates/m were recorded using 1.00 M KCl, at a flow rate of 0.20 mL/min but iodate was not baseline resolved from the void peak. By reducing the concentration of the eluent to 0.100 M, efficiencies of up to 39 900 plates/m could be obtained at 0.35 mL/min. By employing a linear gradient ranging from 0.05 to 1.00 M KCl the ions dissolved in distilled water or a salt water matrix could be baseline separated in less than 3 min at a flow rate of 2.50 mL/min.

Item Type: Article
Keywords: inorganic ions • ion-exchange chromatography • matrix elimination • polymer monolith
Journal or Publication Title: Journal of Separation Science
Page Range: pp. 2598-2604
ISSN: 1615-9306
Identification Number - DOI: 10.1002/jssc.200800205
Additional Information: The definitive published version is available online at: http://interscience.wiley.com
Date Deposited: 19 Aug 2008 22:34
Last Modified: 18 Nov 2014 03:48
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