Library Open Repository

Indirect photometric detection of anions in nonaqueous capillary electrophoresis employing Orange G as probe and a light-emitting diode-based detector

Downloads

Downloads per month over past year

Blanco-Heras, GA and Breadmore, MC and Johns, CA and Hutchinson, JP and Hilder, EF and Lopez-Mahia, P and Haddad, PR (2008) Indirect photometric detection of anions in nonaqueous capillary electrophoresis employing Orange G as probe and a light-emitting diode-based detector. Electrophoresis, 29 (14). pp. 3032-3037. ISSN 0173-0835

[img] PDF
044.pdf | Request a copy
Full text restricted

Abstract

A method based on indirect photometric detection (IPD) in CE employing a blue LED (473 nm) as a light source and the highly absorbing (478 nm) anionic dye, Orange G, as the probe ion was developed for the sensitive analysis of inorganic and organic anions. The use of nonaqueous solvents was examined as a simple way to reduce the adsorption of the dye onto the capillary wall and to thereby improve the baseline stability. The benefits of this approach were confirmed by experiments using BGEs in methanol (MeOH) and DMSO in which superior baselines were obtained relative to those achieved using aqueous electrolyte systems. A range of commercial LEDs was tested to improve the detection performance, with a difference of 25% in sensitivity being observed between the best and worst performing LED. The final system (4 mM Orange G, 0.05% w/v hydroxypropylcellulose (HPC), 20 mM triethanolamine (TEA) in pure MeOH) exhibited stable baselines and very low LODs (0.10–0.18 mM) for a test mixture comprising nine inorganic and organic anions. These values represent a two- to six-fold improvement over previous studies and the proposed method provides the most sensitive IPD method for the determination of anions using CE published to date. RSDs for ten replicates were in the ranges of 0.42–0.62% for migration time, 1.41–3.46% for peak area and 3.20–5.78% for peak height.

Item Type: Article
Keywords: Anions • Dye • Indirect photometric detection • Light-emitting diode • Nonaqueous capillary electrophoresis
Journal or Publication Title: Electrophoresis
Page Range: pp. 3032-3037
ISSN: 0173-0835
Identification Number - DOI: 10.1002/elps.200800116
Additional Information: The definitive published version is available online at: http://interscience.wiley.com
Date Deposited: 21 Aug 2008 21:54
Last Modified: 18 Nov 2014 03:48
URI: http://eprints.utas.edu.au/id/eprint/7312
Item Statistics: View statistics for this item

Repository Staff Only (login required)

Item Control Page Item Control Page