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Fingerprinting and pedigree analysis in Eucalyptus globulus using RAPDs

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Nesbitt, KA and Potts, BM and Vaillancourt, RE and Reid, JB (1997) Fingerprinting and pedigree analysis in Eucalyptus globulus using RAPDs. Silvae Genetica, 46 (1). pp. 6-11. ISSN 0037-5349

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Abstract

RAPDs were used to confirm clonal fidelity and distinguish
individuals of varying degrees of relationship in Eucalyptus
globulus. RAPD variation was examined amongst ramets
derived from 10 ortets which were either full-sibs, half-sibs, or
unrelated, as well as their parents. Genetic similarity amongst
samples was calculated from 117 RAPD bands using the simple
matching coefficient. RAPD variation within clones was trivial
compared to the variation found even between full-sib clones.
Genetic similarity decreased with pedigree distance. The
pedigree of the material was successfully displayed by UPGMA
clustering of the genetic similarity matrix, with parents
generally clustering with their offspring. In a second experiment,
120 RAPD bands allowed the successful discrimination
of selfs from their intra- and interprovenance half-sib outcrosses
and again genetic similarity decreased with pedigree
distance. These results clearly indicate that RAPDs have great
potential for estimation of outcrossing rates and pedigree
analysis.

Item Type: Article
Keywords: Key words: Eucalyptus globulus, RAPD, fingerprinting, breeding system, pedigree analysis, genetic distance.
Journal or Publication Title: Silvae Genetica
Page Range: pp. 6-11
ISSN: 0037-5349
Additional Information:

BM Potts.Free online access to the archives of SILVAE GENETICA

Date Deposited: 22 Sep 2008 02:46
Last Modified: 18 Nov 2014 03:49
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