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Early ovule development following self- and cross pollination in Eucalyptus globulus Labill. ssp. globulus

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Pound, LM and Wallwork, MAB and Potts, BM and Sedgley, M (2002) Early ovule development following self- and cross pollination in Eucalyptus globulus Labill. ssp. globulus. Annals of Botany, 89. pp. 613-620. ISSN 0305-7364

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Abstract

The study was conducted to identify the self-incompatibility mechanism in Eucalyptus globulus ssp. globulus. Controlled self- and cross-pollinations were conducted on individual flowers from three mature trees that had self-incompatibility levels of 76, 99·6 and 100 %. Flowers were harvested at 4, 6 and 8 weeks after pollination. Embryology was investigated by bright field microscopy on material harvested at 4 and 6 weeks after pollination. Fertilization had taken place at 4 weeks after pollination with zygotes and free nuclear endosperm visible. There was a greater proportion of healthy, fertilized ovules in the cross- compared with the self-pollination treatment, and approx. half the ovules examined from both pollen treatments were not fertilized or were degenerating. By 6 weeks after pollination a few zygotes were starting to divide. The number of healthy, fertilized ovules was still greater in the cross-pollination treatment, but the number of healthy fertilized ovules was lower in both treatments compared with 4 weeks after pollination, and many ovules were degenerating. Fertilized ovules were significantly larger than non-fertilized or degenerating ovules and this difference was detectable by eye at 6 and 8 weeks after pollination. The mechanism of self-incompatibility appears to have both late pre- and post-zygotic components.

Item Type: Article
Keywords: Eucalyptus globulus ssp. globulus, Tasmanian blue gum, self-incompatibility, controlled pollination, bright field microscopy, embryology, fertilization, ovule, zygote
Journal or Publication Title: Annals of Botany
Page Range: pp. 613-620
ISSN: 0305-7364
Identification Number - DOI: 10.1093/aob/mcf089
Additional Information: BM Potts. © The definitive version is available at http://www.sciencedirect.com
Date Deposited: 02 Oct 2008 06:01
Last Modified: 18 Nov 2014 03:49
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