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Single-component eluents for quasi-linear pH gradients in weak cation exchange columns

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Vakshtein, MS and Ivanov, AV and Nesterenko, PN and Tessman, AB (2006) Single-component eluents for quasi-linear pH gradients in weak cation exchange columns. Journal of Liquid Chromatography, 29 (4). pp. 485-496. ISSN 1082-6076

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Abstract

The formation of decreasing internal pH gradients in carboxylic cation-exchange columns, with a single buffering component in the eluent, was investigated. Two types of carboxylic cation exchangers (hypercrosslinked polystyrene and polymethylmetacrylate based) were used. To develop a pH gradient, the column was pre-equilibrated at pH 7.5 with Tris buffer, followed by washing with different organic acid solutions, including acetic, oxalic, tartaric, citric, and glutamic acids. The smoother quasi-linear gradients in the pH range from 7.5 to 3.5 were obtained with 0.2-1 mM citric acid as eluent. The noticeable role of ionic strength of the starting buffer and of the eluent on the profile of pH gradient was demonstrated. The optimal pH gradient profiles were obtained at equal ionic strengths of both mobile phases.

Item Type: Article
Keywords: Chromatofocusing; Decreasing pH gradient; Weak cation-exchange column; Carboxylated sorbents; Single-component eluent
Journal or Publication Title: Journal of Liquid Chromatography
Page Range: pp. 485-496
ISSN: 1082-6076
Identification Number - DOI: 10.1080/10826070500474071
Additional Information: The definitive published version is available online at: http://www.tandf.co.uk/journals
Date Deposited: 19 Sep 2008 01:20
Last Modified: 18 Nov 2014 03:50
URI: http://eprints.utas.edu.au/id/eprint/7563
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