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Olfactory ensheathing cells are attracted to, and can endocytose, bacteria

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Leung, YK and Chapman, JA and Harris, JA and Hale, DM and Chung, RS and West, AK and Chuah, MI (2008) Olfactory ensheathing cells are attracted to, and can endocytose, bacteria. Cellular and Molecular Life Sciences, 65 (17). pp. 2732-2739. ISSN 1420-682X

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Abstract

Olfactory ensheathing cells (OECs) have been shown previously to express Toll-like receptors and to respond to bacteria by translocating nuclear factor-kB from the cytoplasm to the nucleus. In this study,we show that OECs extended significantly more pseudopodia when they were exposed to Escherichia coli than in the absence of bacteria (p=0.019). Coimmunoprecipitation showed that E. coli binding to OECs was mediated by Toll-like receptor 4. Lyso- Tracker, a fluorescent probe that accumulates selectively in lysosomes, and staining for type 1 lysosomeassociated membrane proteins demonstrated that endocytosed FITC-conjugated E. coli were translocated to lysosomes. They appeared to be subsequently broken down, as shown by transmission electron microscopy. No obvious adherence to the membrane and less phagocytosis was observed when OECs were incubated with inert fluorescent microspheres. The ability of OECs to endocytose bacteria supports the notion that OECs play an innate immune function by protecting olfactory tissues from bacterial infection.

Item Type: Article
Keywords: olfactory ensheathing cells - chemotaxis - endocytosis - innate immunity - ultrastructure
Journal or Publication Title: Cellular and Molecular Life Sciences
Page Range: pp. 2732-2739
ISSN: 1420-682X
Identification Number - DOI: 10.1007/s00018-008-8184-1
Additional Information: The original publication is available at www.springerlink.com
Date Deposited: 16 Sep 2008 05:57
Last Modified: 18 Nov 2014 03:50
URI: http://eprints.utas.edu.au/id/eprint/7582
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