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Sequence variation in two lignin biosynthesis genes, cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase 2 (CAD2)

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Poke, FS and Vaillancourt, RE and Elliott, RC and Reid, JB (2003) Sequence variation in two lignin biosynthesis genes, cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase 2 (CAD2). Molecular Breeding, 12. pp. 107-118. ISSN 1380-3743

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Abstract

Cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase 2 (CAD2) are genes which may influence variation in lignin content and composition within plants. Sequence variation within these genes may be responsible for changes in enzyme activity and/or specificity, which could cause variation in lignin content or composition. This study examines sequence variation within these two genes in Eucalyptus globulus, an important species used in pulp and paper-making. Twenty-one single nucleotide polymorphisms (SNPs) were identified in the exons of CCR, of which nine were neutral mutations and 12 were missense mutations. Six of the missense mutations affected highly conserved amino acids within the protein sequence of CCR. Eight SNPs were identified in the CAD2 exons, six of which were neutral mutations and two which were missense mutations. One of the missense mutations affected a highly conserved amino acid within the protein sequence. In addition, 32 SNPs were identified in the CCR introns along with four insertion/deletions and two polyA length variation regions. Polymorphism affecting highly conserved amino acids may alter enzyme function and this molecular variation may be linked to variation in lignin profiles. Selecting positive alleles which produce favourable lignin profiles would be advantageous in tree breeding programs.

Item Type: Article
Keywords: Candidate genes, eucalypt genetics, Eucalyptus globulus, lignin, single nucleotide polymorphism, forest tree breeding
Journal or Publication Title: Molecular Breeding
Page Range: pp. 107-118
ISSN: 1380-3743
Identification Number - DOI: 10.1023/A:1026006828721
Additional Information: The original publication is available at www.springerlink.com
Date Deposited: 22 Sep 2008 23:13
Last Modified: 22 Sep 2008 23:13
URI: http://eprints.utas.edu.au/id/eprint/7618
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