Library Open Repository

Use of coupled open-tubular capillaries for in-line ion-exchange preconcentration of anions by capillary electrochromatography with elution by a transient isotachophoretic gradient

Downloads

Downloads per month over past year

Hutchinson, JP and Macka, M and Avdalovic, N and Haddad, PR (2004) Use of coupled open-tubular capillaries for in-line ion-exchange preconcentration of anions by capillary electrochromatography with elution by a transient isotachophoretic gradient. Journal of Chromatography A, 1039 (1-2). pp. 187-192. ISSN 0021-9673

[img] PDF
337.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.

Abstract

Open-tubular capillaries have been joined together for use in on-column ion-exchange preconcentration of anions by capillary electrochromatography (CEC) with elution by a transient isotachophoretic gradient. This involved the coupling of a preconcentration capillary and a separation capillary using a PTFE sleeve. Such coupling allowed precise lengths of differently coated capillaries to be joined in-line to form a single multi-mode column. The different segments could be tailored to optimize a separation by either altering the length of each segment to precisely manipulate the amount of stationary phase present or by changing the internal diameter of each segment to alter the phase ratio in the chromatographic column without affecting the path length for UV detection. In this work, a segmented in-line capillary was used in conjunction with a fluoride–octanesulfonate discontinuous electrolyte system to increase the number of anions that could be preconcentrated and separated. Quaternary ammonium functionalised latex particles were used for creating the preconcentration segment and the separation segment was coated with poly(diallyldimethylammonium chloride). This allowed the detection of trace anions in drinking water and in situ sampling of river water for the analysis of trace inorganic anions. The repeatability of producing the quaternary ammonium functionalized latex-coated segments was assessed and the effect of segmentation on peak efficiency was investigated.

Item Type: Article
Keywords: Electrochromatography; Capillary columns; Isotachophoretic gradients; Water analysis; Environmental analysis; Coupled columns; Inorganic anions
Journal or Publication Title: Journal of Chromatography A
Page Range: pp. 187-192
ISSN: 0021-9673
Identification Number - DOI: 10.1016/j.chroma.2004.03.073
Additional Information: The definitive version is available at http://www.sciencedirect.com
Date Deposited: 03 Oct 2008 02:28
Last Modified: 18 Nov 2014 03:51
URI: http://eprints.utas.edu.au/id/eprint/7698
Item Statistics: View statistics for this item

Repository Staff Only (login required)

Item Control Page Item Control Page