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Interference of some matrix ions in cation-exchange chromatography
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Ion chromatographic analysis of ions in samples containing complex matrix composition strongly depends on the on-column co-processes caused by sample matrix components. In the present publication studies of different separation phenomena in cation-exchange chromatography are described. The studies were performed at ‘non-linear’ chromatographic conditions, when the concentration of matrix (interfering) ions significantly exceeded the concentration of the eluent ions. During the research work, the processes already identified in anion-exchange chromatography, i.e. self-elution, on-column change of the eluent and sample-induced micro-gradient elution were used to explain the chromatographic behavior of alkaline and earth-alkaline cations when samples with high matrix cation concentration were analyzed. When present at higher concentrations, the ammonium cation was found to cause self-elution (NH4+ fraction) as well as on-column eluent neutralization due to its ability to diffuse into/back from the porous core of the stationary phase (NH3 fraction). Co-elution of a matrix component and analytes of interest caused spectroscopic interferences that additionally influenced the peak shape of each individual analyte.
|Keywords:||Alkaline metals; Alkaline earth metals; Inorganic cations; Matrix effects; Gradient elution, sample-induced|
|Journal or Publication Title:||Journal of Chromatography A|
|Page Range:||pp. 89-96|
|Identification Number - DOI:||10.1016/S0021-9673(03)00976-2|
The definitive version is available at http://www.sciencedirect.com
|Date Deposited:||03 Oct 2008 06:36|
|Last Modified:||18 Nov 2014 03:51|
|Item Statistics:||View statistics for this item|
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