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A new and selective capillary column coated with cationic diazacrown ether for separation of organic and inorganic anions by capillary electrophoresis

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Mori, M and Tsue, H and Tanaka, S and Tanaka, K and Haddad, PR (2003) A new and selective capillary column coated with cationic diazacrown ether for separation of organic and inorganic anions by capillary electrophoresis. Electrophoresis, 24 (12-13). pp. 1944-1950. ISSN 0173-0835

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Abstract

A new coated capillary has been introduced for capillary electrophoretic separation of anions by using a positively charged diazacrown ether with a 12-membered ring. A positive charge spread over the inner capillary surface led to a substantial anodic electroosmotic flow (EOF) over the range of migrating buffer of pH 2-11. Under the optimum conditions of 25 mM phosphate buffer at pH 7, the diazacrown-coated capillary showed a successful simultaneous separation of 7 inorganic anions and 13 aromatic anions (including positional isomers) in less than 15 min. The migration times of the sample anions and EOF marker for consecutive runs on a single column were highly reproducible, giving a relative standard deviation of 1%. Theoretical treatment of the migration behavior clearly demonstrated that ion association between the diazacrown and analyte anions is strongly dependent on the nature of the functional groups of anions (e.g., sulfonate groups > carboxyl groups) and the number of negative charges (e.g., trivalent anions > divalent anions > monovalent anions) on the analyte.

Item Type: Article
Keywords: Capillary electrophoresis • Cationic diazacrown ether • Coated capillary • Ion association • Organic and inorganic anions
Journal or Publication Title: Electrophoresis
Page Range: pp. 1944-1950
ISSN: 0173-0835
Identification Number - DOI: 10.1002/elps.200305503
Additional Information: The definitive published version is available online at: http://interscience.wiley.com
Date Deposited: 10 Oct 2008 05:42
Last Modified: 18 Nov 2014 03:51
URI: http://eprints.utas.edu.au/id/eprint/7753
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