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Modification of ion chromatographic separations by ionic and nonionic surfactants
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New findings are reported on simple ways to modify an ordinary HPLC column to obtain efficient ion chromatographic (IC) separations. Permanently coating a column with an ionic surfactant is known to produce an effective column for IC. We now show that incorporation of a nonionic surfactant in the coating, or coating in separate layers, results in a dramatic reduction of ion retention times and gives sharper peaks. Dynamic coating by incorporating a small amount of an alcohol, diol or zwitterion in the aqueous mobile phase permits good separations of alkanecarboxylic acids. A mobile phase containing a quaternary ammonium cation and a zwitterion anion provides excellent separations of common anions on a silica C18 column. An aqueous eluent containing a mixture of a zwitterion 4-(2-hydroxyethyl) acid and methanesulfonic acid can be used in conjunction with a standard cation exchange column. After passing through a membrane suppressor, the mobile phase has a slightly acidic pH, permitting divalent transition metal ions (as well as others) to be detected by conductivity.
|Keywords:||Surfactants; Inorganic anions; Inorganic cations; Organic acids; Coated columns; Dynamic coating|
|Journal or Publication Title:||Journal of Chromatography A|
|Page Range:||pp. 21-31|
|Identification Number - DOI:||10.1016/S0021-9673(03)00114-6|
The definitive version is available at http://www.sciencedirect.com
|Date Deposited:||13 Oct 2008 01:29|
|Last Modified:||18 Nov 2014 03:51|
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