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Measurement of detector linearity and effective pathlength in capillary electrophoresis

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Johns, CA and Macka, M and Haddad, PR (2003) Measurement of detector linearity and effective pathlength in capillary electrophoresis. LC-GC Europe, 2003 (May). pp. 290-293. ISSN 1471-6577

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Abstract

Detection linearity and effective pathlength are the two most important parameters for the performance of every photometric detector. Both parameters have been determined experimentally for a high-sensitivity detection cell (HSDC) and an extended light path capillary (ELPC) used in capillary electrophoresis. A series of solutions of increasing sodium chromate concentration was flushed through the capillary under pressure. A plot of sensitivity versus absorbance yielded both the linear range and effective pathlength of the detectors. The upper limit of the linear range was found to be 2190 mAU for the HSDC and 1490 mAU for the ELPC. The effective pathlengths were found to be 1068 and 128 μm, respectively.

Item Type: Article
Journal or Publication Title: LC-GC Europe
Page Range: pp. 290-293
ISSN: 1471-6577
Additional Information: © 2003 Advanstar Communications. All rights reserved
Date Deposited: 13 Oct 2008 02:00
Last Modified: 18 Nov 2014 03:51
URI: http://eprints.utas.edu.au/id/eprint/7773
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