Library Open Repository

Comparison of aqueous and nonaqueous carrier electrolytes for the separation of penicillin V and related substances by capillary electrophoresis with UV and mass spectrometric detection

Downloads

Downloads per month over past year

Hilder, EF and Klampfl, CW and Buchberger, W and Haddad, PR (2002) Comparison of aqueous and nonaqueous carrier electrolytes for the separation of penicillin V and related substances by capillary electrophoresis with UV and mass spectrometric detection. Electrophoresis, 23 (3). pp. 414-420. ISSN 0173-0835

[img] PDF
276.pdf.pdf | Request a copy
Full text restricted
Available under University of Tasmania Standard License.

Abstract

A method for the determination of penicillin V together with its impurities and by-products formed during biosynthesis, using capillary electrophoresis (CE) with UV and electrospray-mass spectrometric (ESI-MS) detection is presented. Aqueous and nonaqueous electrolytes containing 20 mM ammonium acetate were investigated to determine their suitability for the separation of these analytes. These carrier electrolytes were optimized with respect to the pH and the solvent/s used (water, methanol, acetonitrile, ethanol and isopropanol) and it was shown that although the nonaqueous electrolytes offered unique separation selectivities, the best results in terms of selectivity and sensitivity were obtained for the aqueous system. Finally, the applicability of this method for the analysis of a mixture representative of a real fermentation broth was demonstrated using an aqueous carrier electrolyte with both UV and ESI-MS detection.

Item Type: Article
Keywords: Capillary electrophoresis; Electrospray-mass spectrometric detection; Penicillin
Journal or Publication Title: Electrophoresis
Page Range: pp. 414-420
ISSN: 0173-0835
Identification Number - DOI: 10.1002/1522-2683(200202)23:3<414::AID-ELPS414>3.0.CO;2-K
Additional Information: The definitive published version is available online at: http://interscience.wiley.com
Date Deposited: 20 Oct 2008 22:54
Last Modified: 18 Nov 2014 03:52
URI: http://eprints.utas.edu.au/id/eprint/7841
Item Statistics: View statistics for this item

Repository Staff Only (login required)

Item Control Page Item Control Page